Quality Of In Vivo Produced Embryos From Boran And Holstein Breeds With Alternate Sire And Dam Combination In Dairy Animals Under Controlled Environment

The difference in dairy breed type can determine the success of bovine embryo transfer by influencing the quantity and quality of embryos during in vivo embryo production. This is particularly true since response to the superovulatory hormone treatments varies because of breed and doses of the hormone. In this study, the quantity and quality of embryos were evaluated for Boran and HF*Boran (50% and 75% cross breed) dairy cows alternatively using semen from HF and Boran sires. Total of 42 HF*Boran and HF cows were used to study superovulatory response, 33 HF*Boran cows for evaluation of estrus behavior and 36 HF*Boran and Boran dairy cows for evaluation of embryo quality as three separate experiments. In experiment one, three different dosages of Pluset® (FSH hormone) (500 IU, 650 IU and 800 IU) were evaluated for their best response. In experiment two, types and strength of estrus behaviors were characterized as predictor of superovulatory responses. In experiment three, the donor dams and sires were alternatively used from different breeds to evaluate breed effect on qualities of embryos flushed on Day-7 post AI. In experiment one, the superovulatory response rate was significantly higher (P

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