Escherichia Coli0157h7 Prevalence And Sources Of Contamination Of Cattle Meat At Municipal Abattoir And Butcheries As Well As Its Public Health Importance In Debre Brerhan Ethiopia

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The study was carried out from November 20 13 to April 2014 with the objecti ves of determining rnthe prevalence and source of contamination of E. coli 0157: H7 from municipal abattoir and rnbutcher houses, to assess the public health importance of this organism and to lest the drug rnsensitivity pattern of the isolates in Oebre-Berhan. A total of 1,132 samples from abattoir and 40 rnsamples from butcher hOllses were examined. Samples from abattoir included hide swabs, rninternal and external carcass swabs. fecal sample, intestinal mucosal swabs and environmental rnsamples (carcass in contacts, i.c. knife, workers hand, and clothing). Likewise, the samples from rnbutcher houses were swab from carcass, butcher man's hand, cutting board and knife. In rnaddition, stool samples from suspect individuals visiting Debre-Berhan referral hospital were rncollected. The samples were transportcd in Buffered peptone waler to laboratory of the rndepartment of Biological science, Oebre Berhan University in ice box and scrutinized according rnto international standards. From 220 animals slaughtered, a total of 1100 sampl es were taken and rnonly 6 (0.54%) were found to be positive of £. coli 0157:117. E. coli 0157:H7 was isolated from rn2 (0.91 %) of hide swab samples, 3 (1.36%) of feces samples and I (0.45%) of intestinal mucosal rnswab. There were no isolate found from 32 environmental sampl es, 20 stool samples and 40 rnswab samples from butcher shops. Prevalence of E. coli 0157:H7 was significantly associated rnwith the cleanness of the hide (P=0.036). All E. coli 0157:H7 isolates were then checked for rntheir susceptibility pattern against ten selected antibiotics. The isolates were completely suscepti ble to 7 antibiotics tested, namely Tetracycline (TE, 30 Ilg), Chloramphenicol (C, 30Ilg), rnSulfamethoxazole-Trimitoprim (SXT, 251lg), Ciprofloxacine (eIP, 5Ilg), Nalidixic acid (NA, rn30llg), Kenamycine (K . 30llg) and Sterptomycine (S, JOllg) but they were resistant to rnAmoxicillin (AML, 25 Ilg). Though, the prevalence of E. coli 0157:1-17 in present study is low, rnthe impact of the pathogen should not be underestimated.

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Escherichia Coli0157h7 Prevalence And Sources Of Contamination Of Cattle Meat At Municipal Abattoir And Butcheries As Well As Its Public Health Importance In Debre Brerhan Ethiopia

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