Malaria is caused by protozoan parasites of the genus Plasmodium. It is one of the leading causes of illness and death in the world. It is a major health problem in Ethiopia. The aim of this study was to determine ten-year slide positive rate of malaria at Motta Hospital. A retrospective study was conducted to determine the prevalence of malaria parasite infection from peripheral blood smear examinations in Motta Hospital. The case notes of all malaria cases reported between 2006 and 2015 were carefully reviewed and analyzed. Within the last decade (2006-2015) a total of 37180 blood films were requested for malaria diagnosis in Motta Hospital and 4289 (11.53%) microscopically confirmed malaria cases were reported with a fluctuating trend. Regarding the identified Plasmodium species, Plasmodium falciparum and vivax accounted for 60.1% and 34.9% of malaria morbidity respectively and mixed infection of both P. faliciparum and P. vivax was 5%. Malaria was reported in all age groups and both sexes. But the 15-29 year age group and males were more affected. The prevalence of malaria parasite infection between males and females was not significantly associated (P. value>0.05), whereas the prevalence among age groups was significantly associated (P. value