Urinary tract infection (UTI) is caused by an array of pathogens. But, UTI of bacterial origin isrnby far the most common one affecting millions of people worldwide. The present retrospectivernstudy was conducted to assess the status of UTI in Entoto Number One Health Center (ENOHC),rnAddis Ababa. The objective of this study was to assess yearly trend, and the distribution of UTIrnbased on gender and age among patients who visited ENOHC from 2013 to 2017. Data collectedrnfrom the health centerâ€™s record office from 1 February to 31 May 2018, processed and analyzedrnusing SPSS version 20.Chi-squared test was used to test age, gender-based variations, andrnyearly differences at p-value â‰¤0.05,Totally 1805 (6.0%) were examined by urinalysis andrnmicroscopy and were positive for bacterial UTI. The study shows that yearly UTI prevalence inrnthe study area increased successively for the last five years. It was 3.7, 6.0, 7.5, 8.0 and 8.3% inrn2013, 2014, 2015, 2016, 2017 From a total positive 1293(71.6%; 95% CI: 69.4-73.7%) werernfemales and 512(28.4%, 95% CI: 26.3-30.6%) males with female to male ratio of 2.5:1.Thernmean age was 34.33 (95% CI: 33.72-35.26) and standard deviation 16.189. The prevalence ofrnUTI based on age group, the highest proportion (26.3%) was noted in the age group 15-24rnyears, and the lowest (0.4%) among 0-4 age grouped. From the highest age distribution of 15-rn24, 19.2% were females and 7.1% males. The proportion of UTI-positive patients among femalesrnwas significantly higher. Females, especially women of child bearing age were highly affected.rnOverall, the findings demonstrate that UTI remains a growing challenge in the health center.rnRegular monitoring and devising appropriate intervention mechanisms is required.