Medicinal plants have not been well studied, tested, or documented in Dejen district. Most of therninformation is still in the hands of the traditional healers. This study was carried out betweenrnNovember 2017 and May2018 to explore ethnobotanical information on the use of medicinalrnplants in Dejen district. A total of 100 informants were interviewed using semi structuredrninterview, group discussion& field observation. A total of 72 plant species were reported forrntheir medicinal uses in the study area. From those 48 species were recorded for the treatment ofrnhuman ailments, 17 species for livestock and 7 species for the treatment of both human andrnlivestock ailments. The majority of the plants (49%) were found to be harvested from the wildrn(natural) habitat. The most frequently used plant parts were leaves (31%) followed by rootsrn(22%) . The most widely used method of preparation was crushing (29.6%) of the different plantrnparts followed by squeezing (24.1%). In the study area shrubs (52.5%) were the dominant plantrnforms followed by herbs (23.5%). The common route of administration recorded was oral (35%)rnfollowed by dermal (28%). Informant consensus showed that Zehneria scabra is the mostrnutilized species by the community. Agricultural expansion, fire wood, construction, drought werernthe major threats to plants in general and to medicinal plants in particular in the study area.rnAwareness creation, and running insitu and exsitu conservation of medicinal plants is mandatoryrnto ensure sustainable use of the traditional medicinal plants in the area.