Effects Of Some Culture Conditions On In Vitro Propagation Of Aframomum Corrorima (braun) P.c.m. Jansen)

Aframomum corrorima is one of the common native crops of Ethiopia which is used as spice and for medicinal purpose. It is an important cash crop having good export potentials and its seeds are used as carminative, purgative and tonic. Major production limitation of A. corrorima is lack of high yielding improved varieties with improved agronomic practices like multiplication and the slowest seed germination. The objective of this study is to investigate the effect of medium volume, density of explants and some nutrient concentration in in vitro propagation of A. corrorima. Capsules of A. corrorima were collected in Oromia region from Jimma zone Goma Woreda at Chocho Keble. The seed were washed by tap water, then sterilized with 70% ethanol for one minute followed by 5% Sodium hypo chlorite for 25-minute sand washed three times with sterilized distilled water and cultured on semi sol id MS culture medium. Seeds started to germinate at 5th weeks and completed at 11th weeks. This difference is might be happened due to maturation difference of seeds in different capsules and also might be un equal effect of KH2PO4 on seeds when soaked it. The maximum mean shoot number recorded during initiation stage was (1.55 ± 0.11), obtained on full strength medium containing 1.5 ml/l BAP. The maximum mean shoot number (3.80 ± 0.23) was obtained on the medium containing two explants per culture vessel. In all treatments the growth regulator hormone used was 1.5 ml/l BAP. The highest mean root number (14.50 ± 0.34) was obtained on one third salt strength supplemented with 1.5 ml/l IBA. Up on acclimatization 90 % were survived in the green house. It can be concluded that A. corrorima can be successfully micro propagated from seeds by minimizing transportation and area consumption cost.

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