Tobacco is a complex chemical matrix, and annlY~ls or one or it, components.rnnicotine. is from the filct that varities arc inherently dim-.rent in their niclIilnl' c()ntlmts.rnVarities retain their relative rankings in nicotine content despite difrerences in wcathcrrnconditions and locations. III this study, nicotine content of virginia tobacco or I:thiopiarnhas been investigated. 24 different samples were collected from Bilate and Shoa- Robit orrnSouthern and Central parts of the country. Technique that has been applied to the analysisrn"I' ni;;((inf: in Virginia tobacco was a typical single-hcaln spectroscopy. Alit" datarnproce:;sing by Spce!.ronic Gencsys-2 was used te determine thc actual nicotine conlent inrnVirginia tobacl'o.