Retrospective cohort study (1997-2002) was conducted to determine the prevalence,rnincidence and risk factors for Herpes simplex virus type 2 (HSV-2) infections among factoryrnworkers at two sites in Ethiopia. Among enrolled participants in a cohort study of HIVrnincidence and disease progression, 1222 (71.3%) were males and 491 (28.7%) were females.rnThe median age was 35 years for males (range, 19-62) and 33 years (range, 19-46) forrnfemales. The serologic status of each stored plasma sample for HIV and syphilis has beenrnroutinely determined. Antibody to HSV-2 was detected by using the HerpeSelect TM 2 ELISArnIgG, Focus Technologies, USA. The HSV-2 prevalence at enrollment was 41.1%, 57.2%rnamong females and 34.6% among males (p