Back ground: Laboratory service is a key component of Focused Antenatal Care (FANC) to identify lifernthreatening conditions of maternal and child health. Limited capacity of health facilities to providernadequate laboratory services remains a major problem for the quality of FANC service delivery.rnObjective: To assess the status of focused antenatal carernlaboratory services using availability,rnfunctional status and client satisfaction at public health facilities in Addis Ababa, Ethiopia.rnMethods: Institution based, descriptive cross-sectional study was conducted from April to May 2015.rnBoth quantitative and qualitative designs were used. Four hundred and twenty two study participantsrninterviewed on satisfaction towards FANC laboratory services by using structured questionnaire. Arnqualitative data was collected by In-depth interview of providers. To assess availability and functionalityrnof the FANC laboratory, observation check list was used. Data were coded and analysed by using SPSSrnVersion 20 software. Logistic regression model was also used to examine the effect of selected variablesrnon clientsâ€™ satisfaction with laboratory services. P-Value less than 0.05 were taken as statisticallyrnsignificant.rnResult: Only 5(38.5%) out of 13 visited health facilities reported the availability of all types of basicrnFANC laboratory investigations. Comparing the availability of individual tests in the study facilities,rnurine dipstick, urine miscopy and stool examination were available in all institutions. However, only 7rn(53.8%) of the facilities reported the availability of Hepatitis B virus screening test. Rapid syphilis/RPRrntest was found in 10 (76.9%) facilities. All laboratory facilities had at least one or more basic FANCrnlaboratory tests interruption more than a day within the last one year due to shortage of reagent andrnelectric power disruption. Additional analysis of pregnant women's satisfaction toward FANC laboratoryrnservice showed, only 56.9 % (240/422) of pregnant mothers were satisfied with FANC laboratoryrnservices. Those clients who waited less than one hour to get laboratory results were six times more likelyrnto be satisfied than those who waited more than one hours [AOR = 6.03; CI (2.86-12.67)].rnConclusion: Majority of the health facilities reported incomplete FANC laboratory investigations.rnHepatitis B screening test was the lowest available test in the study facilities. Furthermore a slightlyrnhigher maternal dissatisfaction toward FANC laboratory service was observed compared with otherrninstitutional based study in similar settings