Agreement Between Enzyme Linked Immunosorbent Assay With Rapid Plasma Reagin And Treponemapallidumhaem Agglutination Assay For Screening And Diagnosis Of Syphilis At National Blood Bank Service In Addis Ababa Ethiopia

Background: Serological diagnosis of syphilis is based on demonstration of specific treponemalrnantibody and non treponemal antibodies (reagin) in body fluids. Accurate and reliable testing is arncritical step in ensuring the diagnosis of syphilis and the safety of blood transfusion service.rnPerformance agreement between syphilis tests is not documented well in Ethiopia.rnObjectives: To determine agreement between Enzyme Linked Immunosorbent Assay with RapidrnPlasma Reagin and TreponemapallidumHaemagglutination Assay for screening and diagnosis ofrnsyphilis.rn.Methods:Laboratory based Cross sectional study was carried out from January 2016 to Junern2016 at National Blood Bank Service Addis Ababa, Ethiopia. All positive (One hundred andrnninety) and 190 negative samples were used which stored in NBBS (National Blood BankrnService) laboratory from July 2015 to Dec 2015 (total 380 samples). Systematic randomrnsampling method was used to select negative samples .The data was analyzed by SPSS versionrn20 software. The percentage agreement and κ value were compared; the sensitivity, specificity,rnpositive predictive value and negative predictive value of the ELISA and RPR were calculated. Prnvalue less than 0.05 were taken as statistical significant.rnResults:From 190 ELISA positive sera, 151 (80%) were confirmed as positive by TPHA, 39 (20rn%) were found to be false positive. 59 (39%) of samples were positive and 92 (61%) were falsernnegative by RPR. From 190 ELISA negative sera all were negative by RPR and TPHA. Thernsensitivity, specificity, PPV and NPV of ELISA was 99.9%, 85%, 79 % and 100% respectively.rnWhile RPR was 62%, 99 %, 100% and 63 % respectively. Percent agreement of ELISA withrnTPHA was 90% and corresponding kappa value was substantial 0.795. Percent agreement forrnRPR was 66 % with kappa value of 0.375 which was a fair agreement.Chi-square result with prnvalue 0.000 which is statistical significant indicate the association between categorical variables.rnConclusion The study demonstrated that ELISA was very sensitive. There was good agreementrnbetween ELISA and TPHA. RPR was with high specific and weak sensitive results. There wasrnpoor agreement between RPR and TPHA

Get Full Work

Report copyright infringement or plagiarism