The three major soil-transmitted helminths (STH), i.e., Ascaris lumbricoides, Trichuris trichiurarnand hookworm (Necator americanus/Ancylostoma duodenale) are among the most widespreadrnparasites worldwide. Despite the global expansion of preventive anthelminthic treatment, thernmonitoring of anthelminthic efficacy is not a common phenomenon. The objective of this studyrnwas to assess the efficacy of a single 400 milligram dose of albendazole (manufactured byrnKhandeiwal Laboratories Pvt. Ltd) against the major STHs using a standardized protocol. InrnMarch 2011, fresh stool samples were collected from 298 students enrolled in one primaryrnschool located in Wondo Genet, Southern Ethiopia. Efficacy was assessed based on cure ratern(CR) and the fecal egg count reduction (FECR) using quantitative Kato Katz technique. Thernprevalence of any STH infection among school children was 74.8%, showing that the communityrnrepresents a high risk community. High CR of the drug was observed for A. lumbricoides (97%)rnand hookworm (95.3%). The efficacy of the drug was low (42.3%) for T. trichiura. The FECRrnwas also very high for hookworm (93.3%) and A. lumbricoides (90%), but low for T. trichiurarn(25%). The findings suggest that 400 mg single dose of Albendazole is effective againstrnhookworm and A. lumbricoides but a single-dose of ALB is unlikely to be satisfactory for T.rntrichiura parasite treatment. For this parasite a repeated dose regime of ALB for threernconsecutive days is likely to be more appropriate. Alternative drugs are mebendazole (singlerndose 500mg), pyrantel+oxantel (single dose 10mg/kg) and albendazole + ivermectin. This willrnhave better effect in reducing prevalence and intensity of infection in school children due to thernparasite.rnKey words: Helminths, Albendazole, Efficacy, Cure Rate, FECRrnCreated