Sweet potato, (Ipomoea batatas), is vegetatively propagated crop. Due to the following cycles ofrnpropagation, viruses are accumulated, which seriously affects the sweet potato yield and quality. Thernobjective of this work was to produce virus free sweet potato planting materials through meristem culturernand chemotherapy. Stem cuttings of four cultivars were collected from Awasa Agricultural ResearchrnCenter and planted in a greenhouse at Science faculty AAU. These samples were tested for the presence ofrnsweet potato viruses using NCM-ELISA. From the infected plants, meristems were taken and cultured tornmake them free from viruses. In addition, shoots from all varieties were taken and treated with ribavirinrnunder different concentrations for one month. NCM-ELISA test was carried out to confirm the success ofrnvirus elimination. The in vitro propagated plants from meristem culture were found to be virus free.rnSamples treated with 10 mg/l ribavirin, gave positive results for SPFMV. On the other hand, 20 mg/l andrn30 mg/l, treated samples gave negative results for the tested viruses.rnIn this study, micropropagation system in four sweet potato varieties using meristem culture wasrnestablished. MS medium with 3% sucrose, 0.7% agar and different concentrations and combinations ofrnplant growth regulators adjusted at a pH of 5.8 was used throughout all experiments. Twelve treatmentsrnfor initiation of shoots from meristems were done. Significant differences in response to initiation existedrnamong the varieties (p â‰¤ 0.05). Among all combinations tested, 1 mg/l BAP, 2 mg/l GA3 and 0.01 mg/lrnNAA gave maximum shoot initiation percentage (90% for Beletech, 70% for Koka-12 and 56.7% forrnOgensegen). Shoot initiation for TIS-8250 was not possible in all of the combinations but callusing wasrnhighly evident.rnShoot multiplication was dramatically improved by subculturing of the initiated shoots onto differentrnconcentrations of BAP enriched medium. The highest significant mean numbers of shoots per shootedrnexplant (8.0 for Beletech and 6.7 for Koka-12) were obtained in 2 mg/l and 1.5 mg/l BAP containingrnmedium respectively. Subsequently, well formed shoots were rooted on growth regulator free and IBArnenriched medium. The maximum rooting percentage (100%) and the highest mean number of roots perrnshoot (4.2 and 3.7) were obtained from growth regulator free and 0.1 mg/l IBA containing medium forrnBeletech and Koka-12 respectively. Rooted plantlets were acclimatized and 91.4% of acclimatized shootsrnof Beletech and 73.0% of shoots of Koka-12 were survived and successfully established in the greenhouse.rnKey words/Phrases:-Culture initiation, Multiplication, Acclimatization, Chemotherapy, Virus testing,rnSweet potato