The overall objective of this study was the extraction and characterization of the essential oilrnfrom the leaves of Eucalyptus globulus. Eucalyptus globulus of Myrtaceae family was the rawrnmaterial of the experimental research which was collected from Entoto forest, 15 Km North Westrn(NW) of Adiss Ababa. First, the leaf of Eucalyptus globulus was dried through partial sunrndrying. Then the dried Eucalyptus leaves were crushed in cross beater mill with sieve size of 10rnmm and by cutting mill. The sample was sieved using a set of sieves sizes. Next to this, thernexperimental work was carried out by steam distillation set up. A general factorial design wasrnemployed to the extraction process using DESIGN EXPERT 7.0 software and linear regressionrnmodel. This design helped to identify individual effects of extraction time, and particle size, asrnparameter and their interaction in the entire extraction process. In the extraction experiment, thernminimum oil yield of 0.05% was obtained after the extraction time of 1 hour with particle sizernranges of 12.5-20 mm and maximum oil yield of 1.19 % was obtained at optimum extraction timernof 3 hours with particle size ranges of 5-8 mm. This shows that, increasing extraction timern(optimum of 3hrs) and decreasing particle size increases yield. Characterization of the oil wasrnalso carried out. Accordingly the yield was found with 1.4463 refractive index value, 0.90841rnspecific gravity, and optical rotation, 6.31 pH, 3.15 mpas dynamic viscosity, 174 boiling,rn31.725ml/g iodine value, 14 ml/g saponification value, 19.635 acid value and 1,8 cineol(99%)rncomponent were obtained. Finally, the findings of this study will help to indicate the quality ofrnthe Eucalyptus oil which is important in the production of high value essential oils.rnKey Words: Eucalyptus Globules, Steam distillation, Eucalyptus oil, Gc-Ms, 1, 8 Cineol AddisrnAbaba, Entoto