In this study the extraction of chitosan from fish scale was studied and the performance towardsrnfluoride adsorption from aqueous solution was evaluated by batch experiments. The methodsrnused for extraction of chitosan were demineralization, and deproteinization followed byrndeacetylation. Proximate analysis, FTIR and XRD analysis were carried out on the obtainedrnchitosan. Physicochemical properties of obtained chitosan was determined by solubility, waterrnuptake and oil uptake capacity analysis. The water binding capacity and fat binding capacity andrnsolubility were found to be 576%, 219.5% and 83.8%, respectively. Degree of Deacetylation wasrnfound to be 84%. The effects of initial fluoride concentration (10, 15 & 20mg/l), adsorbentrndosage (1, 2 & 3gm) and pH of the solution (5, 7 & 9) were analyzed by Design-Expert versionrn7.0.0 of full factorial versus quadratic model response surface methodology and the optimalrnconditions that gave maximum/efficient removal efficiency were selected. The adsorptionrncapacity of fluoride by chitosan from aqueous solution was found to be 3.248mg/g; and 3gm ofrnadsorbent dosage, pH value of 7 and 10mg/l initial concentration with 98.47% removalrnefficiency were selected as the optimal conditions. The kinetic studies revealed that the data bestrnfits for pseudo second order regression model and the adsorption process followed Freundlichrnisotherm model. The adsorbent after fluoride removal regenerated using 0.1N NaCl aqueousrnsolution. The results indicate that chitosan adsorbent extracted from fish scale has a goodrncapability in the removal of fluoride ions from aqueous solution.