This paper reports a preliminary data on HSV type 2 and type I from a retrospectiverncross sectional study on 1168 (530 female and 638 male) subjects aged 15-66, livingrnat Akaki and Higher 23 (located at the vicinity of All African Leprosy EducationalrnResearch and Training (ALERT)), places around Addis Ababa, Ethiopia. The plasmarnsamples were tested for antibodies against HSV-2 and HSV-l, by using glycoproteinrng (gG2 and gGl) specific ELISA kit. Prevalence of antibodies against HSV type 2rnwas 44.5% (520/1168) in both sexes while the prevalence of antibodies againstrnHSV-l was found to be as high as 94.7% (2311244). HSV-2 serological data isrnpresented in the context of HI V, syphilis, genital ulcer, genital discharge and variousrnsocioepidemiological information. An overall HSV-2 seroprevalence of 64%rn(124/193) in women 35-44 years old and 50% (28/56) in men 45-66 years old wererndetermined. On the other hand seroprevalence of HSV-2 antibodies in womenrnseropositive for HIV-l antibodies was 80% (40/50) versus 47.5% (228/480) amongrnHIV -negatives. Among the HIV positive men, the prevalence was 70% (46/66) andrn36% (206/572) in the HIV-seronegatives. HSV-2 seropositivity was 71.3% (72/101)rnand 45.7% (196/429) in women seropositive and seronegative for syphilis antibodiesrnrespectively. In the men, it was 60% (94/157) and 32.8% (158/481) among thernsyphilis seropositives and syphilis-seronegatives respectively (P < 0.00 I). It wasrnconcluded that being older, HIV seropositive, and syphilis seropositive, werernsignificantly associated (P < 0.05) with HSV -2 seropositivity in both sexes. Highlyrnsignificant (P < 0.05) association was observed between HSV -2 seroposititvity andrnthe various sociodemographic factors examined in both women and men sUbjects.