Genetic Diversity Study Of Ethiopian Faba Bean (vicia Faba L.) Varieties Using Phenotypic Traits Issr And Snp Markers

Faba bean (Vicia faba L.) is one of the earliest food legumes of the world used as source of high protein for human and animal consumption. In Ethiopia, no studies were conducted on faba bean varieties genetic diversity assessment based on molecular markersand this study is designed to fill this gap. The study was designed to reveal the genetic diversity existing among Ethiopian faba bean varieties using phenotypic traits, ISSR and SNP markers. Field experiment was conducted at three locations (Sinana, Agarfa and Selka) for agro-morphological performance, while the molecular part was conducted at Addis Ababa University (ISSR) and UK, LGC genomics (KASP SNP genotyping). A total of 32 faba bean varieties were used for phenotypic and ISSR markers, whereas 16 additional breeding lines were included for SNP markers. A total of 23 phenotypic traits,11 ISSR and 37 SNP primers were used to assess genetic diversity and interrelationship among Ethiopian faba bean varieties. The genomic DNA extraction was made based on modified CTAB method and Oktopure extraction kit method from bulked young leaf sample for ISSR and SNP markers, respectively. The combined analysis of variance over the three locations showed highly significant (p

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