In the present work eleven endemic Aloe species of Ethiopia were cytologically studied.rnThese species include: A. friisii, A. otallensis, A. tewoldei, A. camperi, A. yavellana, A.rnharlana, A. schelpei. A. trigonantha, Aloe species from Bale, A. debrana and A. kefaensisrnSomatic chromosomes were prepared from the root tip meristem using colchcine solutionrnor ice-cold pretreatment, followed by fixation (3:1, ethanol: acetic acid) and enzymernmaceration in pectinase-cellulase solution. The air-dry slides so prepared were stained inrnGiemsa stain for chromosome morphological study or stained in silver nitrate to study thernnumber of nucleoli. This is the first cytological study for A. friisii, A. otallensis, A.rntewoldei and A. camperi. Also all the eleven species were studied for silver staining werernfor the first time.rnThe karyotype analysis has shown that all the species have 2n = 14 chromosomes,rnconsisting of four pairs of large and three pairs of small chromosomes which form arnbimodal karyotype. The maximum number of nucleoli observed varied among the species.rnTwo nucleoli per nucleus was observed in A. kefaensis; four in A. otallensis and A.rndebrana; five in A. tewoldei and A. friisii; and six in A. schelpei, A. trigonantha, A.rnyavellana; A. harlana , A. camperi and Aloe species from Bale. The number of satelliternchromosomes observed generally corresponded to the number of nucleoli. The satellitesrnwere observed on the long arm of long chromosomes and on the short arm of shortrnchromosomes. These occurred in different combinations in different species. Thus, thernspecies were observed to possess all the three pairs, or two long pairs, or one long pair, orrnone long plus one short pairs of chromosomes.rnIt was recommended to extend similar studies to the rest of the endemic species of Aloernand to carry out other studies which involve biochemical and molecular investigations inrnorder to reveal more cytological and genetic variation which would help to elucidate thernphylogenetic and evolutionary relationship among species of Aloe.rnKey words: Endemic Aloe, Ethiopia, karyotype, satellite, nucleoli