In thus work the incidence of candidacies was studied using selected patients of university of Nigeria teaching hospital Enugu analyzed for the presence of candidacies yeast infection. The specimens were cultured on seaboards agar and blood agar and subculture isolated were C aibicans C Tropical C Knusei C Pseudoizo P cals C paraknusei and C stellatoides. The prevalence of candidacies among patients of UNTH wasmonitored in terms of level of occurrence and microbial types. Also the prevalence of candidicesis infection was 40% samples under study.

Title page

Dedication

Acknowledgement

Abstract

Table of contents

CHAPTER ONE

1:1 introduction

1.2 Aim and objectives

1.3 Statement of problem

1.4 Singnificant of study

1.5 Scope of study

1.6 Limitation

1.7 Hypothesis

CHAPTER TWO

2.1 Literature Review

CHAPTER THREE

3.1 Methodology

3.2 Sterilization of methodology

3.3 Population sample

3.4 Preparations of culture media

3.5 Collection of samples

3.6 Identification of isolates

3.7 Antibiotle sensitivity disc testing

CHAPTER FOUR

4.1 Results

CHAPTER FIVE

5.1 Recommendation

5.2 Conclusion

5.3 Reference

5.4 Appendix

LIST OF TABLE

Table I

Age distribution and incident of candidacies. Infection among single and married women of different age group patients unit

Table II

Diameter of zone of l one of inhibition of the organisms with each antibiotic after 24 hours incubation

Table III

Antimicrobial senses pattern of organisms isolated

Table IV

The protein of 80 urine sample detected with aibustixstrips.

CHAPTER ONE

INTRODUCTION

Candeda: This is a yeast like fungi several of which cause disease in man about 90% of infection are to candida albicans, which is normally present in mouth, intestine and veginal it is responsible for the infection in the sites defense mechanisms (carousal 1990)

Candida abbacies usually appears as oval yeast like cell that reproduce by budding however in infected areas. Filamentous hyphare plus pseudolyphae (which consist of elongated yeast cells that remain attached to each other many also be seen the yeast is easily grown at 25⁰c 40 37 ⁰c on sabouraudis glucose agar and if grown on cor a meal agar at 25⁰c the organism can produce many characteristic thick walled chlamydospore Van leeuweuhoed (2 000) candida albicans is measured about 2.5 to 4.0 wm in diameter which can gives rise to pseudomycellun, in the body it has no sexual form the body it has no sexual form in poor medic at temperature of 2b₀^cit produces thick wall and resting cells is about 7 to 17 m in test positive in maminaliam serum it has the ability to split urear. it is pathogenic to rabbits. guinea Pig s and with where it cause abscesses in the kidney when give intran Veroush (Eleke, 2002).

Oral candidacies oral candidacies also called oral thrush) occurs and most frequently in the new born and is probably acquired during passage through and infected vagina the yeast appears as a creamy grade membrane covering the tongue and appears able to produce disease only because of the absence of their resident normal flora (Prescott et al; 1994) if thrush has not occurred by the 3r^dday of life it is likely that it will appear but it should occur which will usually disappear without treatment as other members of the normal flora are acquired

Oral thrush in order children or Adults may occur as a result of endocrine disturbance or ceutaninosis (Particularity a deficiency of rib flauin as a complication of diabeta, as a result of poor oral hyepene or following the administration of corticosteroides or autibutics oral thrush also occur in bottle feed infants which the magnification of my white patches cowering red raw areas of mucous manbrance vanities , vagines cendiass is seen most frequents in women with diabetes, milieus deny pregnamai or following prolonged antibiotic therapy. The prominent symptom is a yellow, milk, veginals discharge yeast cells and pseudo my cell can be found on the mucous membrane and such infections may result in an intense inflammation of the entire ingunal area according to Atlas 199: 13) he state vaginal thrush in pregnant women are susceptible here with production of whish discharge with