The detection of malaria parasite in the blood, which is as the major aim of this project was carried out and experimental data shows the existence of three spp of the parasite in the human blood cell these are as follows:

(a) Plasmodium Falciparium

(b) P. Malarias

Moreover, the last remaining spp, which is Plasmodium Ovale was not observed at all in the human blood cell. Out of 150 specimens collected, 110 were found to be positive indicating that about 74% people in our country Nigeria were living under the shadow of this infectious disease.

Finally, in our findings, it was assumed that the most susceptible group of people to this malaria parasite were the children under the age range of 1 to 10 and the pregnant women in the adult range. This findings was made after staining the thick and thin film using different stains vis: Leishman, Giemsa and field Stains, which enhances a clear observation of morphological appearance of the organisms under the microscopic view.

LIST OF TABLES

TABLE 1 : Different Between Thick and Thin Film

TABLE 2 : Tabulation of the Result Obtained

LIST OF FIGURES

Figure 1 : Stages of Plasmodium Vivax

Figure 2 : Stages of Plasmodium Ovale

Figure 3 : Stages of Plasmodium Malarias

Figure 4 : Stages of Plasmodium Falciparum

Figure 5 : General Life Cycle of Malaria Parasite

Figure 6 : Thick Film of Blood Sample

Figure 7 : Thin Film of Blood Sample

Figure 8 : Techniques of Staining (Field stain A & B)

Figure 9 : Histogram Chart

Title page

Certification

Dedication

Acknowledgement

Abstract

List of table

List of figures

Table of contents

CHAPTER ONE

1.0 INTRODUCTION

1.1 Background Information

1.2 Statement of the Problems

1.3 aim and Objective of the Study

1.4 Hypothesis

CHPATER TWO

2.0 LITERATURE REVIEW

2.1 The Stages of Different Species of Malaria Parasite

CHAPTER THREE

3.0 MATERIALS AND METHOD

3.1 Materials Used

3.2 Sample Collection

3.3 Method of Sample analysis

3.4 Preparation of Blood Film Preparation of buffer Solution to be used with

3.5 Giemsa Stain

3.6 Different Between Thick and Thin Film

CHPATER FOUR

4.0 RESULTS AND DISCUSSIONS

4.1 Results

4.2 Discussion

CHPATER FIVE

5.0 RECOMMENDATION AND CONCLUSION

5.1 Recommendations

5.2 Conclusions

REFERENCES

CHAPTER ONE

1.0 INTRODUCTION

1.1 BACKGROUND INFORMATION

Malaria Parasite is regarded as one of the most serious health problems facing almost the whole world today. It was noted that this parasitic disease is caused by species of poor tozoa called Plasmodium spp. Within the compass of medical environment, malaria was known to be a parasitic disease that gets into human system through the pathogenic bite of an infected female anopheles mosquito, (Knudsen, 1992).

Malaria parasite was also found to be mostly infective to the children under 5 – 6 years and pregnant women, this is because this group of people usually build antibodies which do not prevent the endemic disease of the parasite, but tolerate the victim to a point where it is not consistently lethal (WHO 1986). In the year 1880, Alphonsus Lavaran first observed this parasite at it merizoit stage and was published in the B”Bullentine de Academic de Medicine pariss”. Further findings on this malaria parasite was carried on by Italian doctors which includes Golgi Manson, baslianelli etc. This people observed the mechanism of spreading and the life cycle of the parasite. It was also estimated that 270 million new malaria infection occur worldwide along with 110 million cases of illness and 2 million deaths, where 25% of children’s’ death in Africa are attributed to malaria parasite (WHO, 1986).

Malaria parasite being a parasite, which falls into protozoa called sporazoa. It means that they are spore formers; their life cycle involves an alternation of generation, one, which is sexual, and the other asexual method of reproduction. The sexual and asexual generations in this parasite take place in two different hosts. In the definite host, the parasite carries out the sexual part of the reproduction of which female anopheles mosquito is the host which the asexual part will be carried on in the intermediate host which is in the human blood system (Franklin and Wehrle, 1948).

According to Chees Brough (1987), this plasmodium spp, which is the causative agent of this malaria parasite are of four varieties species that attacks man. They are:

a) Plasmodium vivax, which causes tartian malaria, or vivax malaria

b) Plasmodium malaria, the cause of quatain malaria also called malaria malariae

c) Plasmodium oval that causes oval malaria and finally

d) Plasmodium falciparum, which is the agent of malignant tartian malaria.

With skill and experience these organs and the differentiate disease they cause can be possibly differentiate from each other.

LIFE CYCLE

Among all the mosquitoes that suck blood, it is the female species that has the quality capable of sucking blood, the male mosquitoes are vegetarians, which mean plant feeders, and they feed on the plant juice, which they suck. It is the female mosquito in the genus anopheles that bites man to suck blood, and in the process inject the malaria parasite in the body system.

Infectious mosquito contain in their salivary gland plasmodia, which occur in spindle-shaped form known as sporozoide. Once a mosquito that is parasitise by this organism feeds on the host liver (intermediate host), it injects the sporozoites, these migrate to the host liver where in the parenchymal cells they multiply asexually. This part of cycle is known as exoerythrocytic schizogony, exo-erythrocytic indicates that it takes place outside the red cell, “Schizogony” literally meaning splitting generation. This malaria parasite leaves the liver cells, enter red blood cells, and begin the erythrocytic Schizogony phase. While para-erythrocytic cycle continue in the parachymal cell of the liver. The exceptions being plasmodium falaparum which do not undergo paraenythrocytic cycle. In the red cell the malaria parasite grows in size making use of globin in haemoglobin as its source of protein as amino acid, the residual product collects as a pigment.

Then divisions of the protozoan nucleus occur. At this point, the multinucleated organism inside a red cell is called Schizont or segmenter. And a separate nucleus, which is surrounded by its own cytoplasm, is called a merocoite. The number of the nuclear division and hence the number of merozoite formed varies from species toispecies and this factor is very useful for the identification of the type of species involved. The red cells containing mature merozoite disintegrate, and merozoites thus released invade new red blood cells, becoming trophozoites and the cycle continues.

Because of the significant exception of the plasmodium falciparum for not undergoing para-erythrocytic cycle, different drugs and other control measure are used against this organism in erythrocytic and para-erythrocytic cycle, plasmodium falciparum being relatively easier to control than other species. Mostly often but not all parasitised red blood cells give raise to merozoites. Some plasmodia in red cells ultimately and differently develops either into two sexual forms viz:

The macrogamatocyte (female) or microgamtoclyte (male) or as the asexual form, which continues the cycle in the human system.

The gametocytes develop no further in man and will survive only when they are ingested by female anopueles mosquito. Parenthentically, all the four species of malaria parasite mentioned above can be transmitted from one intermediate host to another by either transfusion of blood or congenitally. The gametocytes ingested by a mosquito undergo further development, from each microgamatocyte, about a dozen or so mobile, flagella like microgametes are formed. Then in the stomach of mosquito there occurs a union of the microgametes and macrogametes resulting in the formation of zygote, which proceeds to develop into an Ookinete.

This Ookinete is a worm like; it migrates to the stomach wall of the mosquito where it transforms to another form known as Oasyst. Then within the Oosyst repeated nuclear divisions occur. The mature Oosyst breaks with the release of thousands of sporozoites into the content mosquito. Sporozoites now migrate to the salivary gland of the mosquito where they are ready again to be injected into the intermediate host (man) for another cycle. The Cycle in the mosquito takes 1 to 3 weeks. In man, the time from mosquito bite to first symptoms and thus the time for the pre-erythrocytic and erythrocytic cycle to occurs vary considerably.

The typical incubation periods in vivax, ovale malaria and palciparum malaria are on the order of 14 – 17, 10 – 14, 28, 8 – 10 days respectively. This period occasionally may be much longer in vivax 16 – 12 mouths and malaria (years) disease condition.