Plectranthus edulis (Vatke) Agnew (syn. Coleus edulis Vatke), is a diploid, dicotyledonousrnplant, belonging to the Labiateae (Lamiaceae) family. It is a major source of food inrnmany parts of Ethiopia and also used as a traditional medicine to treat different diseases.rnThis plant is vegetativelly propagated mainly by tubers. Vegetativelly propagated plantsrnare mostly affected by different diseases. These diseases reduce the yield and results inrnpoor quality crops. The establishment of in vitro plant regeneration system in P. eduils isrnof potential importance to quality improvement and genetic transformation. The studyrnwas conducted to develop in vitro regeneration protocol for P. eduils. For this study, callirnwere induced from leaf explants excised from three-week-old shoots grown on MSrnmedium containing different concentrations of NAA and BAP. The highest (100 %) callusrninduction was obtained on MS medium supplemented with 1.5 mg/l NAA+1.0 mg/l BAPrnand 2 mg/l NAA+0.5 mg/l BAP. Among the different growth regulators combinationsrnused for multiplication, maximum (58.68Â±6.57) mean numbers of shoots were obtainedrnon 0.5 mg/l BAP in combination with 0.4 mg/l GA3. In vitro developed shoots were rootedrnbest with mean root number of 10.15Â±0.95 on MS medium supplemented with 2.0 mg/lrnIBA. All in vitro rooted and 78 % of microshoots were survived in the glasshouse. Thisrnprotocol can be used to improve this plant through in vitro screening and geneticrntransformation.