Chrome tanning is the most and widely used types of tanning process and the wastewaterrngenerated by these industries is a major source of chromium pollution in the form ofrnCr(III)&Cr(VI) which are released into the environment either after treated inefficiently or withrnno treatment and become a cause for many human health problem. Several researchers havernpublished papers to find the solution for removal of Cr(VI) but at its low concentration and thisrnresearch work investigates at high concentration of Cr(VI). Cell suspensions of S.cerevisiae atrnits exponential growth phase were added to 250ml plastic flasks of containing 50ml of YPDrnmedia at pH value of (3,5&8), initial Cr(VI) concentration of (5, 50&100mg/ml) and incubationrntime of(3,5&7 days) and shaken at 200rpm an rnfrom tannery wastewater using S.cerevisiae shows good result after Cr(VI)-contaminatedrnwastewater has been treated at different treatment variables of pH, initial Cr(VI) concentration.rnCr(VI) uptake efficiency of S.cerevisiae increases at low values of pH, Cr(VI) and incubationrntime and decreases at high values of these variables. And it can be conclude that Cr(VI)uptakernefficiency is better at low values of the variables and the optimum conditions obtained by usingrnthe full factorial experimental design are pH value of 4.5, Cr(VI)concentration of 5mg/ml andrnincubation time of 5 days resulting 84.25 % theoretical Cr(VI) uptake efficiency . Cell viabilityrntest assay of this work shows the possibility of Cr (VI) removal at its high concentration usingrnliving cells that are tolerant to high concentration though the efficiency decreases asrnconcentration increases due to inhibition. Bioaccumulation of Cr(VI) by the cell S.cerevisiae isrnanalyzed by FTIR by comparing their IR spectrum of functional groups of the Cr(VI) free andrnCr(VI) loaded S.cerevisiae cell wall that showed change of wavenumbers of biocomponents ofrncell wall of the two cells which confirms the binding of chromium ion with the functional groupsrnof cell wall of S.cerevisiae and produces Cr complex compounds /precipitates. Freundlicrnequilibrium and pseudo second order kinetic model are used for expressing