Production of methane-rich biogas through anaerobic digestion of organic materialsrnprovides a versatile carrier of renewable energy, as methane can be used in replacement forrnfossil fuels in both heat and power generation and as a vehicle fuel, thus contributing tornmitigate the emissions of greenhouse gases and slowing down the climate change.rnIn this study, the potential of Cladodes, which is plate like section, of Opuntia ficus indica, isrnevaluated for its potential in biogas production. Five treatments cow dung alone(T1),rnCladodes alone(T2), and Cladodes used as feed stock mixed with cattle manure in differentrncombinations (at a level of 50:50(T3) ,25:75(T4) , 75:25(T5) ) allowed to digestrnanaerobically at ambient temperature in 2.8litre sand jacketed triplicate batch digesters.rnThe amount of biogas and methane produced over the retention time of 66 days for theserndigesters was compared. The highest total biogas yields were obtained from 75%Cow dung:rn25%Cladodes combination (T5) as 14,183 ml followed by T1 as 13670ml ( 0 .022m3/kg) andrnthe lowest was from Cladodes alone as 6,176ml.rnThe percentage of methane gas obtained from the experiment for treatments T1, T2, T3, T4rnand T5 were 66.33%,53.16%,63.84%,52.1% and 69% respectively. Among all treatments T5rnwas found to produce high methane percent of the biogas. Treatments (T1 and T5) that haverna C:N ratio within the range of 20-30 found to perform better in biogas yield and methanernproduction than that are not. The experimental findings further showed that therncomposition of methane for all treatments were within the range of 50 to 70%. Asrndetermined in laboratory the physico-chemical characteristics of the Cladodes furtherrnrevealed the suitability of the substrate for biogas production and if suitable materials forrnco-digestion, such as manure, are not available, Cladodes can be digested alone.rnStatistical test for the mean difference of dependable variable pH of treatments exceptrnbetween T3 and T5 varies significantly at 0.05 level. The result also showed that the biogasrnand methane content of the gas produced by T5 vary significantly at 0.05 level except withrnT1and T3. Finally, environmental, slurry and foreign currency benefits have been realizedrnthrough fuel replacement value of the biogas produced by the five treatments