Background: Blood donors are required to meet several criteria which are intended to ensurernthat safe blood is made available for transfusion as well as keeping the donors safe. Acute loss ofrniron and both psychological and physiological stress can contribute an increment of bloodrnglucose level after donation. So Screening of blood donors for random glucose level beforerndonation is very important to keep donor’s health.rnObjective: The aim of this study was to determine the increment in random glucose level amongrnblood donors after donation of blood in Wolayita Sodo Teaching and Referral Hospital.rnMethods: A cross-sectional study design was conducted among blood donors in Wolayita SodornTeaching and Referral Hospital from April 1- December 25, 2015. A total of 269 volunteer bloodrndonors (30 female and 239 male) who came to the hospital were interviewed. Data wererncollected by using structured and pre-tested questionnaire. 6ml of blood sample was drown :(rn3ml before and 3ml after) donation blood was tested for random glucose levels using fullyrnautomated Clinical Chemistry Analyzer (BS 200). Descriptive statistics was used to calculaternsome variables in the study. Paired t test and 95%CI was used to assess the significance.rnResults: Most of the study participants were in the age range of 21-31years. About 29.7% of thernparticipants donated blood multiple times. About 6.4% of the participants experienced moderaternto severe post-donation reaction. The mean value of blood glucose concentration in manyrnsubjects after blood donation was significantly higher than the value before donation,rn9.9mmol/L±6.4 Vs 5.6±6.4mmol/L :( P0.05).rnConclusion and recommendation: In general the result of the present study showed thatrnRandom glucose level is elevated after blood donation (P