Biological Degradation Of Feather Keratin

A total of 165 bacteria were isolated Ii'om samples collected Ii'om Mojo TannelY and AbijatarnLake, and 17% were found to possess feather-degrading capacity. Two best keratinolytic strainsrnwere selected Ii'om the 28 feather degrading isolates and tentatively identified as Bacillus lentilSrnstrain Kr-07 and Streptomyces sp. strain Kr-43. The optimal growth period, pH and temperaturernfor the two strains were 96h, 7.5 and 37"C respectively. The proteases of Bacillus lentils strainrnKr-07 and Streptomyces sp. strain Kr-43 were inhibited by PMSF and by EDTA indicating thatrnthey are serine-type and metallo-type proteases respectively. Protease kr-07 displayed its optimalrnactivity at a temperature of 35°C and at a pH on.5, while protease kr-43 at a temperature of 40°Crnand at a pH of7.5.In the presence of 5mMCaChprotease Kr-07 showed optimal activity at 45°Crnat the optimal pH, while protease Kr-43 exhibited optimal activity at 55°C at its optimal pH.rnProtease Kr-43 displayed 85.3% relative activity at 4°C indicating that it is cold active proteasernand hence it could have potential application in the detergent industries, especially in cold areas.rnThe strains can utilize feather, fish wastes, and hom wastes. Therefore, they could be potentiallyrnimportant in the development of waste treatment methods in the biotechnological industriesrninvolved in the processing ofkeratinacious wastes.rnKey words: Keratin, Keratinolytic bacteria, Protease, Keratinase, and Degradation.

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