Study On Comparative Diagnostic Techniques Of Fasciolosis In Slaughed Ruminants At Elfora Export Abattoir Debre Zeit Ethiopia

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A stud y was conducted at EL FO RA Export Aballoir. Dcbrc·Zeit in order to compa re the rnd iffe ren t di agnos ti c tec hniq ues in nl1 11 in anl fa sc iolo sis. T he results of standa rd faeca l rnsedim entat ion techni que fo r nuke egg quant ifi cat ion was compa red to th a t of an l!llzymc rnlinked immu nosorbcll t assay (indirect ELI SA ) les t and liver inspect io n as we ll as res u lts of rnSenllTI enzyme activ ities. Out o f th e 324 call1 e , 350 sheep and 385 goats s laughtered at rnELFORA Export Abatto ir , 125 (38.5%), 4 ( l.l-Jo~o) and 2 (0.5 10'0) were found 0 be posni-e rnfor lesions of fasc io losis, re specti ve ly duri ng post mortem in specti on. Dur ing faeca l rnexam ination 108(33%) , 4( 1. 14%) and 2(0. 5 1%) wc re pos iti ve for fascio la eggs in catt lc ,sheep rnand goats respec tively. Furt her examinatio n on 13.f ran d om ly selected samples from eanlc rnrevea led that 75%, 29°0 and 4 1% we re positi'e lIs ing sero logical, fa ecal and postmortem rnexamination techn iques. respec ti vel y. In sma ll nuninant s . the preva le nce of fasclo losls IIS rnlow botb in coproscopy a nd indirec t ELI SA testes: on ly one o ut of 68 sheep was positive rncoproscopicall y a nd J we re posi tiv e sero logi cally: and one a liI of 22 goats tested was po::,itl e rnboth at coproscopy and se rology exam ina ti ons. Ti ll S indicates the high scmlllvity of ELISA rncompared to the others techniques in d iagnos ing fascio los is. The o 'era ll assessment indicatl!s rnthe relatedness of faeca l examina ti on result w ith ELISA find ings. though thc test agreement I::, rnweak ( Kappa 0.236). SimJiariy. there was a strong relationship between faecnl e'ammatlon rnand postmortem findings o f liver lesions, Kappa value . P=0.758). Out of the 5-l canle Hh rnknown li'er lesions of fasc iola 33 (6 1. 1%) were halng e levated Iccls of GGT than l10nmil rn'aluc for caule. m support of the above statement, our findmgs regardmg the kvcl of GGT rnsuggest its association wi th li ver lesions encounte red at postmortem examination. The k cl of rntest of agrccment between the liver exa mination and GGT determination as howc er rnmoderate (Kappa=0.332). On the other hand the level of LOll has shown to bc gcnerall) rnele atcd both in negative as we ll as pos itive anllnals ror live r lesions mdicatmg the absence of rnrelationship between liver les ions and level of LDI L The use 01 LliSA IS found to be a more rnreliable d iagnostic lest than coproscopy in detecting fasciolosis III ruminants

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Study On Comparative Diagnostic  Techniques Of Fasciolosis In Slaughed  Ruminants  At Elfora Export Abattoir Debre Zeit Ethiopia

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