Mycobacterium bovis (M. bovis) is one of the members of Mycobacterium tuberculosis complex, which infects both animals and man causing mammalian tuberculosis. A cross sectional and case control studies were conducted in Dembia and Gondar districts of North Gondar from September 2005 to April 2006 to determine the prevalence of bovine tuberculosis (BTB) and associated risk factors and assess the sources of infection of human tuberculosis (HTB). In the cross sectional study, cattle from extensive (310) and semi intensive (315) production systems were tested with the comparative intradermal tuberculin test (CID). In the case control study design, 50 human tuberculous patients (cases) and a similar number of patients visiting the hospital for some other health problems other than tuberculosis were interviewed about their hygienic practices, feeding habits and cattle management practices that would contribute to the transmission and maintenance of the disease. From each of the cases and control groups, 200 cattle owned or attended by them were tested with the CID test. Of all 1025 animals examined, 21% were positive. In the cross sectional study, assuming doubtful reactors as negative, prevalence was higher (16.8%) in semi-intensive production system than extensive (11.6%) and this difference was significant (p