The family of Tephritidae are economically important agricultural pests. Among them Bactrocera is the most devastating genus known that damages fruits and lads to economic losses. To control these pests female targeted attractants are essential. Previous work has shown that females of fruit flies are attracted to protein baits. The objective of the present work was to identify volatiles from a mixture of five different lures (brewery yeast, bakerâ€™s yeast, torula yeast, GF-120 and anamed) that elicit antennal response of our experimental species. The experiment was carried out on five species of fruit flies (Ceratitis capitata, Bactrocera dorsalis, Bactrocera zonata, Bactrocera cucurbitae and on Bactrocera oleae). We scored the similarity and dissimilarity of these species in the antennal response to volatiles from mixed fermentation and protein lures using gas chromatography electorantennography detection (GC-EAD). Nine compounds were identified that elicited an antennal response. Of these nine compounds in C. capitata, seven compounds in B. zonata, six compounds in B. dorsalis and B. cucurbitae and two compounds in B. oleae gave an antennal response. The active compounds were identified using gas chromatography coupled to mass spectrometry (GC-MS). In olfactometer assay flies were attracted to brewery yeast and a mixture of fermentation and protein lures made. The findings are discussed in the potential of developing a bait that attracts these fruit flies.rnKey words: protein baits, antennal response, GC-EAD, GC-MS, Bactrocera and Olfactometer