Coffee processing industries generate huge amount of wastewater. The effluent is characterizedrnby a lot of sludge, high amount of organic load and acidic nature due to fermentation process.rnDue to this, it contains high value of COD, BOD, TS and other contaminants. This effluent whenrndisposed in natural water, it contaminates aquatic animals and plants environment. So, care hasrnto be taken to ensure the quality of the releasing waters should be reduced below the prescribedrnstandards. There are many physical and chemical treatment methods available for the removal ofrnpollutants but all these methods have problems associated with secondary effluent, hazardousrnand harmful end products, high energy consuming, non-economic etc. These problems can bernovercome by the use of biological treatment methods which are simple, eco-friendly and efficientrnwhere complete removal of the pollutants is possible. Cognizant of this, the researcher wasrninitiated to study the treatment of wastewater effluent from coffee industry in the specific area byrnusing anaerobic Pseudomonas florescence and Escherichia coli bacteria as mixed culture andrnwith gypsum (CaSO4.2H2O). In this study, the wastewater value of BOD5, COD, TS andrnneutralization of the PH reduction was achieved. The results show that the treatment reducedrnBOD5, COD and TS from its initial concentration of 320.26 mg/l, 1261 mg/l, and 3545 mg/l torn35.66 mg/l, 97 mg/l and 68 mg/l respectively. The treatment in the study shows 87.74% reducedrnof BOD5 load, 92.02% reduced of COD and 98.01% reduced of TS from the initial load usingrnoptimization of the treatment method. Therefore, it can be concluded that mixed culture bacteriarnof Pseudomonas florescence and Escherichia coli in combination with gypsum as a newrneffective treatment having potential for BOD5, COD and TS reduce from the effluent treated.rnThe mixed culture of microorganisms is capable to reduce the contaminants that made thernapproach cost effective, time saving compared with other results found by other authors withoutrnmixed culture. The microorganisms included in the study can be applied for the treatment ofrneffluents containing the multiple contaminants.