In recent years the advancement of technology results not only development but also,rnenvironmental risks due to the use of chemicals, catalysts and environmentally hazardousrnmaterials moreover coasty. In spite of this, the use of biotechnological principles and products inrnthe industrial sector is mandatory and expected to with stand the current problems. ThoughrnMicrobial alkaline proteases are stimulating tremendous interests in the research and enzymernmarket. Hence, the aim of this thesis was the use of eggshell and membrane as a substrate for thernproduction, optimization and detergent compatibility of alkaline protease using Bacillus subtilisrnmojavensis in submerged fermentation. Fermentation process for enzyme production wasrnconducted using, four factors with three levels were used and 29 experiment had been studied. Thernoptimum or maximum alkaline protease production was obtained at a temperature of 37 rnC,rneggshell concentration of 20 %, pH of 9.0 and incubation time of 48 h with a maximum activity ofrn214 U/ml. Optimum parameters for the production of alkaline protease was found using designrnexpert optimizer at 9.08 pH, 39.74 rn0rnC, 19.78% eggshell and 48 h of incubation time were obtained.rnAlkaline protease enzyme was produced for further analysis such as, characterization andrndetergent compatibility of the enzyme. Enzyme activity was conducted in a pH range of 6-12, withrnmaximum activity obtained at pH 10. It showed that more than 93.97% its original activity wasrnretained using buffers pH ranging from 8-10, with the optimum activity at pH 10.0. Overallrnalkaline protease enzyme was stable between pH 8.0-10.0 attain 90%, 100% and 94% of residualrnactivity. Its minimum residual activity was attained at pH value of 6.0 which is 39.6% of its originalrnactivity. Alkaline protease was found to be very active at all temperatures tested between 30 rn0rnCrnand 80 rn0rn0rnC, alkalinernprotease was stable and retained more than 74% of its maximum activity. From 1M to 2.0M ofrnNaCl concentration, exhibits > 97% of optimum activity was recorded at pH 8 and 10. Whereas;rnoptimum stability was attained at these concentration range between 1.0 - 3.0 M NaCl. At pH 8.0rnresidual activity were raising as concentration increases and constant after 2.5 M. The detergentrncompatibility study was conducted. Results increasing the concentration of enzyme increases therndegradation of blood stain. Therefore, alkaline protease enzyme has compatibility to industrialrnapplication including detergent as an alternative. rnC with maximum activity at 60 rn0rnC. Within temperature range of 40rn0rn0rnC - 70rn0rn0rnC alkalinernprotease was stable and retained more than 74% of its maximum activity. From 1M to 2.0M ofrnNaCl concentration, exhibits > 97% of optimum activity was recorded at pH 8 and 10. Whereas;rnoptimum stability was attained at these concentration range between 1.0 - 3.0 M NaCl. At pH 8.0rnresidual activity were raising as concentration increases and constant after 2.5 M. The detergentrncompatibility study was conducted. Results increasing the concentration of enzyme increases therndegradation of blood stain. Therefore, alkaline protease enzyme has compatibility to industrialrnapplication including detergent as an alternative.