Bacteria produces Polyhydroxyalkanoates (PHAs), which are macromolecules. They are inclusion rnbodies that build up as reserve resources while bacteria grow under various stress situations. rnPHAs have been chosen as alternatives for the development of biodegradable polymers due to rntheir rapid degradability under natural environmental circumstances. The purpose of this study rnwas to produce reducing sugars from lignocellulosic Napier grass and examine PHA production rnby the impact of temperature, pH, and culture time on growth yields by employing the produced rnreducing sugars as carbon sources using response surface methodology via Box- Behnken design rnBBD). rnBurkholderia Sacchari strain was chosen from more than 300 bacterial strains capable of PHA rnaccumulating strains based on its ability to consume both hexose and pentose sugars and has a rncapacity of accumulating high mass PHA granules inside its cells. At 200 rpm, optimal pH, rnfermentation temperature, and incubation time for the isolate to produce the highest PHA were 7, rn35 Â°C, and 48 hour, respectively. rnWhen hydrolysates of Napier grass was employed as carbon sources and ammonium sulphate as rnnitrogen sources, the strain was able to collect 62.1% PHA from its total biomass. The extracted rnPHAs' FTIR spectra show strong peaks at wavenumbers that are unique to PHAs as Câ€“H, CH2, rnO-H, C=O, and Câ€“O groups. The extract's resemblance appropriateness for bioplastic production rnwere validated by UVâ€“Vis spectrophotometric analysis.