Somatic Embryogenesis Of Finger Millet (eleusine Coracana (l.) Gaertn) Using Seed Explants

Finger millet (Eleusine coracana subsp. coracana) is one of the most important crops in Africarnand India. Finger millet contains different nutrients and is a popular food among diabeticrnpatients in different countries. Finger millet is an orphan crop of developing world on whichrnlittle research has been done. The improvement of this crop is crucial towards abiotic and bioticrnstress tolerance. Tissue culture and transformation techniques are among the approaches tornimprove this crop. Therefore, the objective of this study is to develop an efficient somaticrnembryogenesis protocol for regeneration of different finger millet cultivars. Somaticrnembryogenesis protocol for regeneration of four cultivars of finger millet is developed. For allrncultivars, mature seeds were used for callus induction. After sterilization of the seeds, callus wasrninduced efficiently in different concentrations of 2, 4-D in all cultivars. From 95 to100% callusrninduction were recorded on callus induction medium containing 1.0, 1.5, 2.0, 2.5 and 3.0 mg/l 2,rn4-D. MS-medium supplemented with 1mg/l 2, 4-D resulted in 45 to 58.3% somaticrnembryogenesis and 1mg/l KN in combination with 0.25mg/l NAA was resulted with highest meanrnnumber (15.85±0.63) of shoots and 16.74±1.86 roots were recorded from AAUFM-19.rnrnKey Words: callus induction, Kinetin, NAA, shoot regeneration, somatic embryogenesis

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