Determination Of Some Essential And Non-essential Metals In Fenugreek Seed (trigonella Foenum-graecum L.) Cultivated In Different Parts Of Ethiopia

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Fenugreek (Trigonella foenum-graecum L.) is native to Ethiopia. It is a plant mostly usedrnas a spice and herbal medicine in most countries of the world. Fenugreek has manyrndietary and health benefits. It is used as anti-diabetes, hypercholesterolemia, anti-cancer,rnanti-microbial, against breast cancer, hypoglycemia, antioxidation, laxation, apetiternstimulation, antiulcer, and immunomodulatory, spice in food, etc. In this study, the levelsrnof major (Ca, K, Na, Mg), trace (Fe, Cr, Ni, Zn, Mn, Cu, Co), and toxic (Pb, Cd) metalsrnin seed of fenugreek grown in different regions of Ethiopia were determined by flamernatomic absorption spectrophotometry (FAAS). Wet ashing was used to digest 0.5 grnfenugreek seed flour and was optimized using 1.5 mL of HNO3 and HClO4 acid mixturesrn(5:1 ratio), 30 min pre-digestion time, 45 min total digestion time and 150 0Crntemperature. Thirteen elements were analyzes and their concentrations are listed in thernfollowing ranges and order; Ca (15353–36771 mg/kg) > Fe (6041–18584 mg/kg) > Krn(6789–11517 mg/kg), > Pb (615–2624 mg/kg) > Na (201–1559 mg/kg), > Cd (285–464rnmg/kg) > Cr (3–552 mg/kg) > Ni (31–108 mg/kg) > Mg (31–102 mg/kg) > Zn (15–33rnmg/kg) > Mn(16–28 mg/kg) > Cu (N.D–35 mg/kg) > Co (4–15 mg/kg). A statisticalrnanalysis of variance (ANOVA) at 95% confidence level was used to test whether thernvariation between metal content of fourteen sample means were significant or not. Inrnaddition Pearson correlation coefficient was used to predict the dependence of metalrnlevels of one with another.rnKey words: Fenugreek seed (Trigonella foenum-graecum L.), Major elements, Tracernelements, Toxic elements, Flame atomic absorption spectrophotometry

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Determination Of Some Essential And Non-essential Metals In Fenugreek Seed (trigonella Foenum-graecum L.) Cultivated In Different Parts Of Ethiopia

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