Enzymatic Determination Of Urea At The Membrane-stabilized Liquid-liquid Interface

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The membrane-stabilized water-nitrobenzene interface was used as anrnamperometric (or voltammetric) ammonium ion sensor for an indirect detection ofrnurea. The ammonium ion was produced by the action of watermelon seeds and soyrnbean flour on urea in the aqueous phase (5 mmol/L Li2S04). The detection wasrnbased on the facilitated transfer of ammonium ions by DB[18JC-6. The organic phaserncontained 50 mmol/L DB[18JC-6 and 10 mmol/L EthVTPB. The performance ofrnwatermelon seeds and soy bean flour as soluble urease reagents is described. Thernproblem caused by potassium indigenous to the tissues is discussed. The sensorrnwas applied to the estimation of potassium in the seeds of some plants

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Enzymatic Determination Of Urea At The Membrane-stabilized Liquid-liquid Interface

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