Background: Adolescent growth and development is closely linked to the diet they receive during childhood. The physical and physiological changes that occur in adolescents place a great demand on their nutritional requirements and make them more vulnerable to micronutrient deficiency. Copper and zinc are essential trace elements and nutrients, which have important roles in physiology and disease and as cofactors in many metabolic pathways. Anemia is a widespread condition of known public importance. rnObjectives: The aim of this study is to investigate the status of zinc, copper and anemia among adolescent girls aged 15-20 years old living in Arsi zone, Lode-Hitosa wereda, Ethiopia. rnMethodology: A cross sectional study involving 257 participants was carried out in Lode-Hitosa wereda (Huruta), Oromiya region. An interviewer-administered questionner was used to gather socio-demographic characteristics and dietary pattern. Epinfo was used to enter data from questionnaires. A total of (4ml) blood samples were collected from each study participants. Blood samples of participants were analyzed for hemoglobin (Hb), (ferritin, C-reactive Protein (CRP), and Acid Glycoprotein (AGP)); and serum zinc and copper. Serum Zinc and Copper status were assessed using atomic absorption spectrophotometric method. Data were analyzed using SPSS Version 20 software for Windows. rnResult: The total prevalence of anemia among the adolescent girls was 8.7% (milder form of anemia accounts for nearly all the cases). Iron status was also determined using serum ferritin (SF) values after adjusting for the presence of infection/inflammation based on CRP and AGP values. The prevalence of clinical iron deficiency after adjusting was very low (8.7 %, SF < 12 Î¼g/L). However, a significant proportion had a marginal iron store (41 %, SF< 50 Î¼g/L). The prevalence of infection in the area was about 17 %, of which 0.8%, 1.5 % and 14.8 % were in incubation, early and late convalescence phase, respectively. The prevalence of low serum zinc (