Uptake And Phytoaccumulation Of Chromium At Seedling Stage For Three Commonly Grown Tree Species In Ethiopia

A pot culture culture experiment was conducted in the glass house of Holetta AgriculturalrnResearch Center (HARC) of, Ethiopian Institute of Agricultural Research (EIAR) basedrnon Holetta.The aim of the trail was to study the impact of Cr on seed germination,rnseedling quality and growth, to establish in which part of the plants the metal is stored forrnan eventual use of phyto-stabilization and/or phytoextraction of the metals, and finally tornscreen and recommend the best Cr accumulator tree species for phytoremediationrncapabilities of the three tree species seedlings Viz.Eucalyptus camaldulensis, Millittiarnferruginea, and Ricinus communis respectively. Both biological stable speciation of Crrn(trivalent as Cr2 (SO4) 3.6H2O and hexavalent as (K2Cr2O7) at three concentration levelsrn10,100, 1000 PPm and the control were used.rnThe plant growing media was prepared using, 3-forest soil, 3 local soils, 1 manure and 1rnsand soil. Before sowing the media were mixed in appropriate proportion, moistened,rnsterilized and finally filled into polythen bags of 16cmx20cm diameter and heightrnrespectively, the already filled pots were taken into the green house and laid out with splitrnplot design of CRD (completely randomised design) with 3 replications. The seeds wererndirectly sown on polybags, germination data were recorded however the result was notrnsignificantly different for the two Cr species however it was differing significantly forrndifferent concentration of Cr and for the three tree species respectively .At the end of thernexperiment both seedling growth and quality parameters were assessed but the datarnstatistically revealed that among the parameters height and root collar diameterrnsignificantly differed for the treatments at (P≤0.05) However seedling quality parametersrnwas not significantly different among the same tree species where as there was significantrndifference recorded when the interaction effect of the three tree species was considered.rnxirnFor Cr data analysis the two parts of the plants were separately collected, oven dried,rnweighed, milled, and digested and separately analysed using Atomic AbsorptionrnSpectrophotometer (AAS) (Varian spectra AA-220) with air acethylene flame at 358 ηmrnwith 0.2 spectral slit width. The shoots and roots of Ricinus communis showed a veryrngood Cr storage capacity followed by Millittia ferruginea and Eucalyptus camaldulensisrnrespectively. The shoots and roots accumulate nearly the same amount of Cr in all the treernspecies seedlings respectively. With regard to the uptake of Cr Ricinus communis wasrnalso the most efficient in its storage capacity as it contains the highest level of Cr perrngram of dry matter.

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