Dehairing is one of the highly polluting operations in the leather industry. The lime sulfide dehairing produces large amounts of sulfide, which poses serious toxicity and disposal problems. The aim of this study was to develop enzymatic dehairing of animal skin and determine its potential to eliminate sulfide pollution load. Screening using skim milk containing media protease producing bacterial isolated from soil. The organism produced appreciable level of keratolytic protease using hair as the sole source of carbon and nitrogen. The Isolate grew well on solid state fermentation with wheat bran as a solid substrate. This suggest that the organism produces the enzyme using cheap substrates, hair, its production cost is expected to be low. The isolate produced 2,984 U/g of protease after 96 h of incubation at a moisture level of 71.4 % in the presence of yeast extract. protease BI15 optimally active in the pH range of 8- 10.5 and 45-70Â°C with an optimum pH of 10.0 and 65oC. Under large scale evaluation the enzyme was able to dehairing at a dosage of 7882 U/ml of the enzyme at the inner side of 2 kg of sheep skin pH 10.0 brought about complete removal of hair 12 h at 32oC in tanning drum Indicating their potential for application for dehairing in tanning Industry. Protease BI15 has been obtained in a remarkable elimination of sulfide, reduction of polluting effluent and the collagen was not damaged resulting in a leather of good quality. Thus, offers immense potential for greener mode of dehairing of skins and hides in leather industry coupled with environmental excellence.