The utilization of agro-industrial wastes for the extraction of natural antioxidants such as Ferulicrnacid is currently getting a growing interest by the researchers around the world. This researchrnstudy, therefore, aims at optimizing and characterizing the Ferulic acid extracted from breweryrnspent grains (BSG). Brewery spent grain was first treated with sulphuric acid before it wasrnsubjected to alkaline hydrolysis. The X-ray diffraction analysis of the raw and hydrolyzed BSGrnshowed the evidence for a possible release of phenolic extractives after the hydrolysis. The effectrnof extraction time, extraction temperature, and alkali concentration on the Ferulic acid yield wasrnstudied at (60, 90, and 120 Â°C), (100, 120 and 140 min), and (1, 2 and 3% (w/v)) respectively.rnOptimization of these extraction parameters was carried out by the Box Behnken Design, usingrnthe Response Surface Methodology (Design experiment software version 11). It was observed thatrnthe Ferulic acid yield at different runs ranged from 6.62 mg/100 g BSG to 45.245Â±0.585 mg/100rng BSG. An optimum Ferulic acid yield of 46.85 mg/100 g BSG was observed at 119.83 Â°C, 97.04rnmin, and 2.21% (w/v) NaOH concentration. Both the Fourier Transform Infrared Spectroscopyrn(FT-IR) and High-Performance Liquid Chromatography (HPLC) analysis showed that the BSGrnwas a good candidate for the extraction of the Ferulic acid. All the three main factors consideredrnfor this research study, namely the extraction time, extraction temperature and sodium hydroxidernconcentration had a statistically significant effect on the yield of Ferulic acid extracted.rnSpectrophotometric analysis of the produced Ferulic acid using DPPH radical scavenging methodrnshowed a strong capacity of its antioxidant activity. The result of the present research provides anrnalternative insight into the waste management of brewery spent grains (BSG) through thernproduction of Ferulic acid.