Fossil fuel based plastic raw materials are not renewable which in questioned their sustainabilityrnas well as the plastic products of these raw material is sources of an overwhelmingrnenvironmental problem due recalcitrant to degradation. Hence, there is an urgent need towardrnbio-based plastics research area particularly poly lactic acid (PLA). This study aimed to producernLactic acid which is used as a raw material for bio-based plastic (PLA) by a bacteria Lactornbacillus plantarum using sugarcane bagasse as a carbon source. The effects of glucosernconcentration, time and pH on the lactic acid yield were studied. sugarcane bagasse wasrnpretreated using dilute sulfuric acid as catalyst and a maximum glucose concentration ofrn3.755g/L was found at a temperature of 105rnC, acid concentration of 2.75% and time of 3hrs.Thernfermentation experiments were performed in 250 mL Erlenmeyer flasks as small scale laboratoryrnfermenter containing 100 mL of hydrolysate supplemented with 5g/L of yeast extract and 10mLrnof lacto-bacillus plantarum inoculum. The yield of lactic acid from the fermented broth wasrndetermined using HPLC. Response surface methodology (RSM) approach of Design expertrnsoftware of version 11 was used to analyze the effects of glucose concentration (3.755g/L,rn3.58.g/L and 3.42g/L), pH (4, 6 and 8) and incubation time (10hrs, 20hrs and 30hrs) on lactic rnacid production with their respective ranges keeping the temperature and agitation speedrnconstant. A three-variable, three-level Box-Behnken design was used to develop a statisticalrnmodel to describe the relationship between lactic acid concentration and the chosen independentrnvariables and to observe their effects on the yield using RSM. The model was statisticallyrnsignificant (p Ë‚0.0001), did not show lack of fit (p-value = 0.1141) and The Pred R-Squared ofrn0.9764 is in reasonable agreement with the Adj R-Squared of 0.9956.From the model regressionrnequation developed, the linear terms of glucose concentration, pH and time and pure quadraticrnterm of time as well as interaction of glucose concentration and pH, interaction of glucosernconcentration and time, and interaction of pH and time had positive effect on the response yield.rnOn the other hand pure quadratic terms of glucose concentration and pH had negative effect onrnthe response yield. The maximum yield of lactic acid of 15.79g/L was found at a higher glucosernconcentration of 3.755g/L, at a moderate pH of 6 and at a maximum time of 30hrs.