About two-third of Ethiopian diet consists of Injera, a thin, fermented traditional baked bread, made fromrnthe most popular grain of teff(Eragrostis tef (Zucc) Trotter). Even though it is a nutritious food, the shelfrnlife of injera does not usually exceed 3 days due to mould spoilage. This research was conducted with thernaim to evaluate the effect of biosynthesized silver Nano particles (AgNPs) and Zinc oxide nanoparticlesrn(ZnONPs) in improving injera shelf life. AgNPs and ZnONPs are synthesized from Eucalyptus globulusrnand Calpurnia aurea (Ait.) Benth leaf extracts, respectively. Formation of nanoparticle was confirmed byrncolor change and by characterization techniques (UV-vis spectroscopy, scanning electron microscopyrn(SEM) and X-ray diffraction (XRD). Antimicrobial test on Aspergillus, Pencillinium, and Rhizopus spp.rnwas conducted using disc diffusion method. Then both nano particles were dip-coated on plastic zipperrnbags at different percentages after disinfection with 70% alcohol and dried in an oven at 600c and used forrninjera storage. The shelf life was determined as one day before mold growth appearance. Moisture contentrnand pH was determined by oven and pH meter, respectively. Internal temperature of the package duringrnstorage was measured by infrared spectroscopy whereas pour plate method was used for mold and yeastrncount. Finally, migration level was determined by Microwave Plasma - Atomic Emission Spectroscopyrn(MP-AES). The results from Uvi-vis spectroscopy shows that characteristic peaks observed at 420 and 300rnto 400 nm for AgNPs and ZnONPs, respectively. The XRD data also shows that both synthesized nanornparticles were below 100nm. The SEM image shows that AgNPs was mostly irregular whereas ZnONPsrnwas mostly rod shaped. The result has shown that both of them have significant antimicrobial effect againstrnthe studied fungus. ZnONPs has induced greater inhibition zone than AgNPs, which is caused by differencernin crystallite sizes (ZnONPs = 64.25nm, AgNPs = 84.07nm).The shelf life of the stored injera samples werernincreased significantly based on their concentrations. For both Ag and ZnONPs , the optimumrnconcentration was 50% in which injera shelf life increased for 10 days to 15 days, respectively. Both NPsrnhave no significant effect on moisture, pH and internal temperature of the package during storage. ThernCfu/g of molds and yeast decreased as the percentage of these nanoparticles coated on plastic bagsrnincreased. At 50% concentration the migration was 1.34 and 375mg/Kg for both Ag and ZnONPs,rnrespectively. The migration of both Ag and ZnONPs was relatively high, which was increased withrnconcentration and time of storage. Because, it needs further study and improvements to use these nanornparticles for injera storage. Thus, it would be advisable if these nano particles will be incorporated asrningredients of plastics to decrease this migration problem.