Banana (Musasp.) is cheap and has been grown in most parts of Ethiopia. In its green stage, banana contains mainly carbohydrate especially starch; therefore, it is suited to use as the source of starch and flour. Then banana flour was used for starch isolation using 0.05N NaOH. The gelatinization temperature ranges obtained from differential scanning calorimetry (DSC) were 66.50°C, 75.16°C and 86.54oC for starches. The study was also carried out to determine the biscuit making potentials of cooking banana and wheat flour blends. Accordingly, all the banana flour and starch samples were characterized, and biscuit samples were subjected to laboratory analysis for proximate and minerals using standard methods. Supplementation of wheat flour with cooking banana flour significantly p