The study was conducled at peri-Addis Ababa dislricls of Oromia wilh the aim to assess quality of milk atrneach critical point, and to identify knowledge gap, cOllstraints affecting production to consumption chainrnof milk supply. A total of 1 02 milk producingfarmers al Ho1eta, Sebeta and Sululta districts were selectedrnby using multi-stage purposive sampling method. A total of 60 raw milk samples were collectedrnhygienically from each presumed critical points and examined for their microbial and gross nutrientrncomposition analysis. The main proximate and mineral values evaluated were ash, protein, fat, totalrnsolids and solid not fat; and Ca, Fe, Zn and P, respectively. The main microorganisms assessed were.rnaerobic mesophilic bacterial count, Coliform count, and fecal coliform count, E.coli count, Salmonella,rnStaphylococcus species and Yeast and Mold Counts. About 99% of participants in the areas marketrnwhole milk and 94% of the milk produced per households was sold. About 96.1 and 23% of thernparticipants stated that milk production and marketing in areas maintain household food security andrnprojitable farm activity respectively. The major challenges of milk production and marketing in the areasrnwere; feed shortage, high feed cost, disease, shortage of land for grazing, and price fluctuation duringrnfas ting season, long term contract for milk marketing and milk quality, respectively. Besides, lack ofrntraining for producers, lack of awareness on standard milk and milk product production and marketing,rnlack of aseplic milk handling and use of traditional flavor plants on milk microbial load were majorrnknowledge gap in the areas. The mean ash content for Sebeta, Holeta and Sululta samples collected fromrnfarmers were O. 60Â±0. 032, 0.77Â±0.025 and 0.50Â±0.007 mgl100gm, respectively. There was significantrndifference (p