Conlalllinalion of cereal commodilies by mOlilds and lIIycoloxins resllils in cby IIwller,rnqualily, and nlllrilional losses and represenls a signijicanl hazard 10 Ihe food chainrnAjlaloxins are loxic, hepalocarcinogenic, secondmy lIIeraboliles of Aspergillus speciesrnprodllced in 1II0s1 agricullllral commodilies, slored 01 inappropriale lemperallires and lValerrnaClivilies. Ajlaloxin conlaminalion of lef! in Erhiopia lVas reporled in 1987, indicaling Ihalrnill!;her probabililY of conI aminal ion of leff, sampled Fom differenl parI of Erhiopia.rnThe objeclive oflhis sludy lVas 10 invesligale occurrence of lola I ajlaloxin in lef!prodllced byrncommercial farms of Elhiopia and Ihe ef!ecl of slored lemperallire and relalive humidilY lVasrnalso invesligaled In addilion, KAP sllldy lVas also conducled on OlVners and employees ofrncommercial farmers. A IOled of Eleven commercial lef! producing farms lVere randoml)!rnselecled and, Ajlaloxin B1, B2, C I and C2 were delermined al microgram per kilogramrn(JIg/kg) levels by immllno-ajjinily column clean lip and reversed-phase liquidrnchromatography lVilh fluo rescence defection. The result showed all of Ihem were below thernmaximllm (4 Ilg/kg) ,olerable level of Elhiopion lef! jlollr slandard The highesl AFrnconcenlralion lVas 3.99 JIg/kg and Ihe 10IVesl concenlralions lVas 0.5 JIg/kg. Blil 91%rncOnlaminaled by AFB1 and 45% lVere lInsaJe Jar direcl hllman consumplion as per IhernElhiopian and EU maximllm (2 JIg/kg) ,olerable inlake level and, 27% oj Ihe samples velYrnclose (above 3pg/kg) 10 Ihe 4I'g/kg. 711e highesl ajlaloxin BllevellVasJound in lVesl Cojamrn3.25 Ilg/kg and aj/aloxin B1 is nole delecled in sample form Adam Elhiopia. From Ihernsamples analysec/, 13. 88%, 0%, 8.38% and 77.73% were conlaminaled lV ilh qllanlifiablernconcenlralions of ajlaloxin C2, C1, B2 and 81 respeclively. Ajlaloxin C2 (/3.88%) and BIrn(77.73%) concenlralions lVere relalively higher Ihan Ihe olhers. In addilion, KA P sllldy lVasrncondllcled on commercial farms and il sholVs Ihe/l, Ihe farmers had knolVledge on mycoloxinrnre/a/ed question but their know/edge (0 AFs pracUce 100vards reducing the contaminalionrnlevel of A Fs is nol enough.rnKey word s: Ana toxin, Terr, KAP assessment, Immune-a affinity column