M icronuttien! deficiencies constitute important nutritional and public health problems affectingrnbillions of people globally and the prominent one is iron deficiency. To mitigate iron deficiencyrnnumber of tcchniques used and fortification is the best intervcntional strategy that can be used tornimprove the iron status of population. Recently, there was a concem for extrinsic/geophagy iron forrnbeing sale part of Ethiopian Teffcereal, reported for being bioavailable and alleviated iron deficiencyrnin-vivo study. In addition, it is presumed that on-top of adequate dietary iron intake the extrinsic ironrnconsumption sum effect will surpass the recommended iron intake. However, its toxicological andrnpotential negative interaction on other micronutrient status effect not yet been di scovered. Our studyrnaimed chronic intake of adequate dietaty iron combined with excess dose of extrinsic source iron effectrnon iron, zinc and copper status using in vivo model (weaning male Wistar rats). We fed diet formulatedrnwith adequate dose of iron sulfate (35 ppm)/Control group was compared with five expetimentalrngroups which consumed dietary different dose (adequate/excess) or bioavailability (low/high) indexrniron without or with excess dose of extrinsic soil source iron formulated di et intake and investigatedrnbody weight, liver weight, iron, zinc and copper status for all groups. Excess extrinsic source iron (350rnppm) with adequate dose of electrolyte iron (70 ppm) and excess dose of electrolyte iron (350 ppm)rnformulated diet fed groups showed statistically insignificant growth rate, liver weight, serum iron andrncopper level compared to control group(P