Objective: Considering the fact that elevated serum concentration of cholesterol is a major riskrnfactor for atherosclerotic disease, the effect of consumption palm oil, soya bean oil, Sheno legarnand Niger seed (Nuge) oil-supplemented diet on serum lipid profile of Wistar albino rats wasrninvestigated. These oils were analyzed spectrometrically for their metal (Cu, Fe, Pb, Ni, and Zn)rnlevels by Graphite Furnaces Atomic Absorption Spectrometer. The four samples of vegetable oilsrnwere also examined for peroxide value, acid value, iodine value and % free fatty acid (%FFA).rnMethods: Growing male Wistar albino rats (Rattus novergicus) weighing (165-220g) were usedrnfor this study. The rats were housed individually in cages in groups of 4, and maintained at roomrntemperature (21oC + 2oC) with 12 hrs light and dark cycles. Each group had 6 rats and fed withrn10% palm oil, 10% Soya bean oil, 10% Sheno lega and 10% Niger seed (Nuge) oil -rnsupplemented diets for 12 weeks. The blood samples were drawn by cardiac puncture after 4, 8rnand 12 weeks. The serum levels of Total cholesterol (TC), Triglycerides (TG), Low-densityrnlipoprotein (LDL), and High-density lipoprotein (HDL) were determined at 4-, 8-, and 12-weekrnintervals. The trace metals levels of Copper, iron, zinc, Nickel and lead were determined byrnAOAC 1995 official method 990.05 by using GF AAS, after extracted from low quantities of oilrn(0.3290-0.3590 g ) with a 65% nitric acid solution and Hydrogen per oxide30% using Microwavernassisted digestion and quantization after preparing series of working standard solutions byrndiluting metal stock solution with deionized water to set calibration curve. Physicochemicalrnanalysis peroxide value (PV), iodine value (IV), were determined following the method of thernAOAC official method 965.33 (2000) and AOAC official method(1995) respectively . The acidrnvalue (AV) was determined titrimetrically using the procedure of AOAC official method 940.20rn(1995). Results: In sheno lega group, 4 weeks of sheno lega supplemented fed significantly (p