Anemia is a widespread condition leading to adverse pregnancy outcomes. Ironrndeficiency is often expected to be the cause and thus routine Iron-folic acid (IFA)rnsupplementation is recommended by the World Health Organization (WHO), little or nornstudies have looked at the etiology of anemia in Ethiopia. The role of other micronutrientrndeficiencies such as folic acid, vitamin B12, as well as infections in the etiology ofrnanemia remains unknown. The aim of this study was to investigate the determinantsrn of anemia among third trimester pregnant women (n=208) attending ante-natal care rn(ANC) in Ambo, Ethiopia. A cross sectional study was carried out and a semi-structured rnquestionnaire was used torngather socio-demographic, obstetric and nutritional information. Blood samples of 208rnmothers were analyzed for hemoglobin (Hb) and blood samples of 147 mothers for serumrniron, serum ferritin (SF), transferrin, folate, cobalamin (Vit B12), C-reactive proteinrn(CRP) and α-1-glycoprotein (AGP) levels. Of the women, 23% had mild (10-10.9g/dl)rn and 36% had moderate (7-9.9g/dl) anemia.rnIron deficiency as assessed by serum iron level of