Vitamin A deficiency (VAD) is a public health problem in Ethiopia. It affects vision, growth, tissue differentiation and immune system. Orange-fleshed sweet potato varieties are known to contain high amount of β-carotene and other carotenoids. This study was designed to determine β-carotene retention, mineral composition, antinutrient level and functional properties of orange-fleshed sweet potato varieties. In order to compare β-carotene contents of different orange fleshed sweet potato varieties, to investigate the effect of treatment methods on β-carotene retention, orange-fleshed sweet potato varieties were collected from Hawassa Agricultural and Research Institute. The results showed that β-carotene contents were significantly affected by many factors, and this was demonstrated using the varietes of Kulfo and Tulla. β-carotene contents in orange-fleshed sweetpotatos Kulfo and Tulla grown in the same farming sites in the same area ranged from 400-334.05μg/g and 335.25-280μg/g fresh weight, respectively. Six treatment methods including boiling, steaming, microwave cooking, oven drying, sun drying and post steam-drying were simulated in the study to check their effects on the true retention of β-carotene. Compared to boiling, steaming resulted in much more loss of β-carotene and microwave cooking resulted in the biggest loss of β-carotene among the six treatment methods. The level of retention was significantly different (P