Profile Of Antibody Production Against Schistosoma Mansoni In Mice Immunized With Soluble Adult Worm Antigen

The profile of serum antibody levels against soluble adult worm antigen (SA WA) wasrnexamined in Swiss Albino white mice over a period of 60 days of infection. A group ofrnmice (n= 15) were immunized with 0.2 mg SA WA through the oral route. Seven daysrnafter immunization, the mice were exposed to 100 cercariae of S. lIlansoni. Anotherrngroup (n=15) consisted of un immunized mice that were also exposed to 100 cercariae ofrnS. lIlansoni. Serum samples were pooled from 4 mice within each group at 0, 7, IS, 30,rn45 and 60 days post- infection and the levels of anti-SA W A antibodies determined byrnELISA by using class and sub-class specific monoclonal antibodies. The results revealedrnsignificant difference in the levels of total IgG, and IgG I antibodies in the sera ofrnimmunized mice compared to the unimmunizcd infected mice. In contrast, the IgMrnantibody responses were lower in the sera of immunized mice than in the unimmunizedrninfectedl11ice. Some antibody responses (IgG, IgG I and IgM) began to rise between 15rnand 30 days post-infection. Furthermore, IgG2a, IgG3 and IgE antibody responsesrnpositively correlated with the time of the onset of egg deposition. On the other hand, IgArnand IgE were not detected in the immunized and the unimmunizedmice. The profile ofrnIgG secreting spleen lymphocytes li'otH the two groups of mice showed a similar patternrnof response before the onset of egg deposition (i.e. 45 days). However, the response ofrn, • , I, "_" ( { ( ( .: ( , ( , , , ( , ( , , , ,rnIgG secreting mesenteric lymphocytes yas iJ1iiiHtec:' eqrliPi' , in the'tmftllmiiecl lilice, '[h~llirn, ,

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