Morphological And Random Amplified Polymorphic Dna (rapd) Marker Variation Analysis In Some Ethiopian Drought Tolerant And Susceptible Chickpea (cicer Arientinum L.) Genotypes

Seventeen chickpea genotypes were planted in Randomized block design with four replications atrnDebre Zeit Agricultural Research Center during 2003/2004 cropping season. Ten plants wererntagged for morphological data collection. Both fifteen quantitative and five qualitative traits werernrecorded. RAPD study was conducted at the Genetics Research Laboratory, Department ofrnBiology, Addis Ababa University. The study was conducted to assess the genetic diversity ofrnchickpea using both morphological and RAPD data, heritability and correlation of quantitativerntrait Shannon diversity, analyses of variance, correlation coefficient, heritability and genetic advance,rnphenotypic and genotypic variance and principal component were computed from morphologicalrntraits using SPSS and SAS Software Programs. RAPD data generated from bands recorded wasrnused for computing gene diversity, DNA polymorphism and Jaccard’s similarity via POPGENErnsoftware Shannon diversity index showed the availability of variations within chickpea populations.rnSimilarly, high variation within and between chickpea genotypes was recorded for quantitativerntraits evaluated except for days to flowering and maturity, plant height, canopy width andrnleaflet/leaf. OPC-02 primer used for RAPD marker showed that RAPD can discriminate chickpearnpopulation in diversity analysis of chickpea population. High heritability and with genetic advance recorded for some traits such as hundred seedrnweight, biological yield/plant, grain yield/plant, days to maturity and flowering, number ofrnprimary and secondary branches/plant, number of seeds and pods/plant, harvest index and rootrnbiomass indicated that progress in selection could be high for such traits in chickpearnimprovement Strong and positive significant correlation observed between biological and grain yield,rnbiological yield and number of seeds/plant, biological yield and number of pods, and grain yieldrnwith number seeds and pods/plant, number of pods/plant and number of seeds/plant showed thatrntheir improvement lead to yield improvement in chickpea. As confirmed by the principalrncomponent analysis, these quantitative traits had also high contribution for chickpea variabilityrnand hence, can be used as a source of chickpea improvement. Key words: Chickpea, diversity, RAPD

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