Callus induction and shoot formation from hypocotyls and cotyledons ofrnGuizotia abyssinica Cass. has been achieved in this experiment. Thernfrequency of callus induction was influenced by the concentrations and typesrnof growth regulators. Six to eight-day-old hypocotyl segments andrncotyledons were cultured on MS medium containing different concentrationsrnof NAA, IAA and BAP. Among the various concentrations tested, 0.5 mg/lrnNAA in combination with 1 mg/l BAP was found to be the best forrnmaximum callus induction of hypocotyl explants. Furthermore, 2 mg/l IAArnin combination with 1 mg/l BAP was the best for callus induction ofrncotyledonary explants. Highest percentage of shoot formation (60%) wasrnobtained when cotyledons were cultured on medium supplemented with 3.0rnmg/l IAA in combination with 1.0 mg/ l BAP. Maximum number of shootsrnper explant (20.3) was obtained from medium containing 0.1 mg/l NAA inrncombination with 1 mg/l BAP. The types of explant, growth regulatorrncombinations and genotypes were showed significant effect on shootrnregeneration. The elongated shoots were successfully rooted on mediarnsupplemented with IBA at a concentration of 0.5 mg/l. The shoots werernestablished in soil where 65% of them survived. Morphologically aberrantrnplants were not observed. As plant regeneration protocol is a prerequisite forrngenetic transformations, this protocol can be used for such purposes andrndevelopment of new varieties with desired traits by in vitro selection