Tjlesequence Variation Of Chloroplast Dna Spacer Intergenipacer Region Between Trnt(ugu) And Trnl(uaa) 5exon Of Eragrostis Tef (zucc.) Trotter And E. Pilosa (l.)

This study was carried out to determine cpDNA sequences of the intergenic spacer regionrnbetween trnT(UGU) and trnL(UAA)5' exon of Eragrostis tef and Eragrostis pilosa tornestablish the degree of similarities and variation of cpDNA at the intraspecific andrninterspecific level since this region shows high degree of mutation. The sequence of thisrnregion has been worked out for 35 cultivars of E. tef and two lines of E. pilosa. This wasrnachieved by isolating total DNA by CTAB method and for one cultivar by isolatingrncpDNA and then followed by amplification of three non-coding regions which arernadjacent to each other by PCR using uruversal primers of Taberlet et al. (1991) followedrnby sequencing of the PCR products. From the results of the sequences it has been foundrnthat the 33 cultivars of E. tef and one line of E. pilosa (E. pilosa 30-5) showed nornvariation. But two cultivars of E. tef, namely "Addisse" and "Key murrie", and one linernof E. pilosa (E. pilosa 30-4) showed a deletion of one base. It is the same base that wasrndeleted in all the three. The total number of bases for this intergenic spacer region is 400.rnBut for cultivars Addisse, Key murrie, and E. pilosa 30-4 the total number of bases is 399

Get Full Work

Report copyright infringement or plagiarism